Shanghai Chemtron Biotech Co.Ltd.

Chlamydia Trachomatis and Female Chlamydia Test

Chlamydia trachomatis is an intracellular parasitic microorganism that can invade the eyes, genital tract and other organs. Genitourinary tract infections caused by Chlamydia trachomatis have become a common sexually transmitted disease. It can cause male urethritis, epididymitis, female cervicitis, pelvic inflammatory disease and many other diseases. In particular, it aggravates the development of the disease and causes other complications when it is combined with other pathogens such as Neisseria gonorrhoeae. Chlamydia trachomatis is an important pathogen causing urethritis and cervicitis. Laboratory diagnosis plays an important role because 70%-80% of women and up to 50% of men with infections have no clinical symptoms. The laboratory tests for Chlamydia trachomatis include: 1) cell culture method; 2) antigen detection method; 3) nucleic acid amplification experiments, including PCR, TMA, etc.; 4) other laboratory tests, including serum experiments.

The principle of female chlamydia test:

The female chlamydia test uses a highly specific antigen-antibody reaction and immunochromatographic technique to qualitatively detect whether the clinical specimen contains Chlamydia trachomatis antigen.

During the test, the treated specimen was dropped into the female chlamydia test card loading well. The sample liquid is combined with a pre-coated red chlamydia-labeled Chlamydia trachomatis-specific antibody in a latex binding pad and a blue (green)-colored latex-labeled avidin. The mixture is then chromatographed to the other end under capillary effect. In the case of a positive specimen, the red latex-labeled Chlamydia trachomatis antibody binds to the Chlamydia trachomatis antigen in the specimen to form a red latex antibody-antigen complex. Upon passing through the test zone during chromatography, the complex is captured by another Chlamydia trachomatis antibody immobilized in the test zone to form a sandwich conformate of red latex antibody-antigen-antibody (immobilized on the mold). A red strip will appear in the test area (T). If it is a negative specimen, since the Chlamydia trachomatis antigen is not included, the above-mentioned sandwich complex cannot be formed in the test area (T), and no red strip will appear. A biotin-BSA linker is immobilized in the control zone (C) on the membrane to capture the blue (green) colored latex particle labeled avidin from the mixture. A complex of blue (green) color aggrethin-biotin-BSA (fixed on the membrane) is formed in the quality control zone (C). Therefore, regardless of whether Chlamydia trachomatis antigen is present in a clinical specimen, a blue (green) color band will appear in the quality control zone (C). The blue (green) color band appearing in the quality control area (C) is a criterion for judging whether there are enough specimens, whether the chromatographic process is normal, and also as an actual internal control standard.

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